Queen's motto is Sapientia et Doctrina Stabilitas, generally translated from the Latin as "Wisdom and knowledge shall be the stability of thy times." The phrase is adapted from Isaiah XXXIII.6 and has been in use since the 1850s.

The motto of the Faculty of Law is Soit Droit Fait, a Norman French phrase for "Let right be done." The phrase is traditionally associated with the rule of law in England.

The motto of the Faculty of Medicine is Manu et Corde Medicus, which translates from Latin as "The doctor works by hand and heart."

The motto of Queen's Engineering Society - Quis Dolor Cui Dolium - is more irreverent. It translates as "Who suffers who has a cask of wine?" It was devised in 1963 by Classics professor Tony Marshall after he was approached by Engineering Society executives interested in lending Latin dignity to the society's informal English motto "What the hell as long as there's free beer."

"Princeps Servusque Es" (be a leader and a servant) is the motto of the Office of the Rector, and embodies what the Rector strives to do for Queen's students.