SOP 7.9 - Intradermal Injection in Mice

University Animal Care Committee Standard Operating Procedure

Document No: 7.9

Subject: Intradermal Injection in Mice

Date Issued: February 16, 2012

Revision: 2

Location: Queen’s University

Responsibility: Principal Investigators (PI), Research Staff, Veterinary Staff

Purpose: The purpose of this Standard Operating Procedure (SOP) is to describe the method of oral gavage in mice.


Animal Care Services ACS, Principal Investigator PI, subcutaneous SC, intravenous IV, intraperitoneal IP, intramuscular IM, per os PO, per rectum PR


1. Introduction and Definitions:

The injection methods described within an Animal Use Protocol (AUP) must be followed at all times. The following guidelines provide recommended injection sites, needle sizes and maximum dose volumes. “Good Practices” include:

  • All animals securely and safely restrained prior to injecting.
  • Only three attempts per site should be practiced. If unsuccessful, allow another (trained and competent) person to collect the sample.
  • Use the appropriate gauge needle and volume for the injection site based on the size of the mouse.
  • Before injecting any substance, aspirate first to ensure appropriate placement of the needle (excluding intravenous injections).
  • Always inject with the bevel up on the needle.
  • Always ensure the substances you are injecting are sterile, and use sterile technique.
  • Every animal requires a new sterile syringe and a new sterile needle. With small volumes, it is preferable to dilute the injectable agent to a 50% or less solution to ensure accurate dosing.
  • Disinfecting the skin with alcohol is mandatory for intravenous, intraperitoneal, intramuscular, intradermal, and all biohazardous injections.
  • Choose the appropriate administration route for the substance to be injected.
Recommended Needle Sizes and Volumes
Length of needle: 1/2 inch
Recommended Gauge
Good Practice Volume
(Max per site)
0.05 ml


2. Materials:

  • Sterile syringes
  • Sterile needles (26-27 gauge)
  • 70% alcohol
  • Clippers
  • Injectable solution
  • Anesthetics as required (if using anesthetics: eye lubricant, heating source, Isotonic fluids – see SOP 7.6 Anesthesia in Mice)


3. Procedures:

Intradermal Injection in Mice

Intradermal Injection in Mice

Date New Version
04/25/2016 Triennial Review
03/28/2019 Triennial Review
12/12/2022 Triennial Review, split apart separate injection methods, updated format and reviewed/updated maximum injection volumes


SOP 7.9 - Intradermal Injection in Mice

Download SOP 7.9 (PDF 128 KB)