Standard Operating Procedures

Standard Operating Procedures (SOPs) provide a detailed description of commonly used techniques. SOPs offer investigators an alternative to writing detailed steps in their Animal Use Protocol (AUP). If referenced within an AUP, any deviation from an SOP must be clearly described and justified in the AUP. Approval of the AUP indicates approval of the deviation for that protocol only.

SOPs are approved by the University Animal Care Committee (UACC).  Refer to the appropriate SOP number and title in the procedural description field within the AUP and include necessary details such as compounds, volumes, frequencies, durations etc. (NOTE: SOPs do not need to be attached to your protocol).

SOPs for commonly used species are listed below. Please follow the link to the Queen's UACC approved SOPs for other species. Access requires registration through the Training Coordinator.

SOP development and revision is an ongoing process. Read all SOPs prior to referencing them in your protocols, as they go through periodic revision. If you require an SOP not currently in the database, contact the University Veterinarian.

For technical assistance, contact the Training Coordinator.

Standard Operating Procedures (SOPs)

7.1 Pain Management (Mice)

7.2 Humane Interventions (Mice)

7.3 Aseptic Surgical Techniques (Mice)

7.4 Rodent Post-Operative Care (Mice)

7.5.1 Euthanasia of Adult Rodents via Barbiturate or Injectable Anesthetic Overdose

7.5.2 Euthanasia of Adult Rodents via CO2 Asphyxiation

7.5.3 Euthanasia of Adult Rodents via Overdose of Inhalant Anesthesia

7.5.4 Euthanasia of Rodents via Physical Methods (Mice)

7.6 Anesthesia (Mice)

7.8 Gavage Techniques in Small Animals (Mice)

7.9 Injection Techniques (Mice)

7.10 Submandibular Blood Collection (Mice)

7.10.1 Saphenous Blood Collection (Mice)

7.10.2 Submental Blood Collection (Mice)

7.10.3 Tail Vein Blood Collection (Mice)

7.10.4 Retro-Orbital Blood Collection (Mice)

7.10.5 Cardiac Blood Collection (Mice)

7.11 Retro-Orbital Injections (Mice)

7.12 Rodent Tail Vein Injections (Mice)

7.13 Ear Notching for Identification (Mice)

7.14 Genotyping (Mice)

7.15 Whole Body Perfusion for Small Rodents (Mice)

7.17 Acute DSS Colitis (Mice)

7.18 Chronic DSS Colitis (Mice)

7.19 Clinical Care of Non-Obese Diabetic (NOD) (Mice)

7.20 Manual Restraint of Mice (Mice)

7.22 The Supportive Care of Rodents During Research Procedures (Mice)

7.23 Rodent Ultrasound (Mice)

7.24 Breeding and Weaning (Mice)

7.25 Estrus Cycle Monitoring (Mice)

7.26 Intrafemoral Injections (Mice)

7.27 Hypoxic Study (Mice)

14.1 Management of Controlled Substances

14.2 Transport of Research Animals on Campus

14.3 Clinical Health Reports – Rodent

14.4 Rodent Environmental Health Monitoring

10.1 Pain Management (Rats)

10.2 Humane Interventions (Rats)

10.3 Aseptic Surgical Techniques (Rats)

10.4 Rodent Post-Operative Care (Rats)

10.5.1 Euthanasia of Adult Rodents via Barbiturate or Injectable Anesthetic Overdose

10.5.2 Euthanasia of Adult Rodents via CO2 Asphyxiation

10.5.3 Euthanasia of Adult Rodents via Overdose of Inhalant Anesthesia

10.5.4 Euthanasia of Rodents via Physical Methods (Rats)

10.6 Anesthesia (Rats)

10.8 Gavage Techniques in Small Animals (Rats)

10.9 Injectable Techniques (Rats)

10.10 Submandibular Blood Collection (Rats) (PDF 302 KB)

10.10.1 Tail Vein Blood CollectionIn (Rats) (PDF 204 KB)

10.10.2 Jugular Vein Blood Collection (Rats) (PDF 178 KB)

10.10.3 Cardiac Puncture Blood Collection (Rats) (PDF 181 KB)

10.11 Stereotaxic Surgery (Rats)

10.12 Rodent Tail Vein Injections (Rats)

10.13 Ear Notching for Identification (Rats)

10.14 Genotyping (Rats)

 10.15 Whole Body Perfusion for Small Rodents (Rats) (PDF 158 KB)

 10.17 Acute DSS Colitis (Rats) (PDF 74 KB)

 10.18 Chronic DSS Colitis (Rats) (PDF 75 KB)

 10.19 Morris Water Maze (Rats) (PDF 205 KB)

 10.20 Manual Restraint (Rats) (PDF 490 KB)

 10.22 The Supportive Care of Rodents During Research Procedures (Rats) (PDF 127 KB)

 10.23 Rodent Ultrasound (Rats) (PDF 242 KB)

 10.24 Breeding and Weaning (Rats) (PDF 207 KB)

 10.25 Estrus Cycle Monitoring (PDF 760 KB)

14.1 Management of Controlled Substances

14.2 Transport of Research Animals on Campus

14.3 Clinical Health Reports – Rodent

14.4 Rodent Environmental Health Monitoring

animal facility

All spaces within our animal facility are easy to clean and disinfect, ensuring the health and welfare of the animals in our care.