Standard Operating Procedures

Standard Operating Procedures (SOPs) provide a detailed description of commonly used techniques. SOPs offer investigators an alternative to writing detailed steps in their Animal Use Protocol (AUP). If referenced within an AUP, any deviation from an SOP must be clearly described and justified in the AUP. Approval of the AUP indicates approval of the deviation for that protocol only.

SOPs are approved by the University Animal Care Committee (UACC).  Refer to the appropriate SOP number and title in the procedural description field within the AUP and include necessary details such as compounds, volumes, frequencies, durations etc. (NOTE: SOPs do not need to be attached to your protocol).

SOPs for commonly used species are listed below. Please follow the link to the Queen's UACC approved SOPs for other species. Access requires registration through the Training Coordinator.

SOP development and revision is an ongoing process. Read all SOPs prior to referencing them in your protocols, as they go through periodic revision. If you require an SOP not currently in the database, contact the University Veterinarian.

For technical assistance, contact the Training Coordinator.

Standard Operating Procedures (SOPs)

 7.1 Pain Management (Mice) (PDF 158 KB)

 7.2 Humane Interventions (Mice) (PDF 303 KB)

 7.3 Aseptic Surgical Techniques (Mice) (PDF 84 KB)

 7.4 Rodent Post-Operative Care (Mice) (PDF 223 KB)

 7.5.1 Euthanasia of Adult Rodents via Barbiturate or Injectable Anesthetic Overdose (PDF 134 KB)

 7.5.2 Euthanasia of Adult Rodents via CO2 Asphyxiation (PDF 175 KB)

 7.5.3 Euthanasia of Adult Rodents via Overdose of Inhalant Anesthesia (PDF 150 KB)

 7.5.4 Euthanasia of Rodents via Physical Methods (Mice) (PDF 170 KB)

 7.6 Anesthesia (Mice) (PDF 90 KB)

 7.8 Gavage Techniques in Small Animals (Mice) (PDF 204 KB)

 7.9 Injection Techniques (Mice) (PDF 103 KB)

 7.10 Blood Collection (Mice) (PDF 430 KB)

 7.11 Retro-Orbital Injections (Mice) (PDF 156 KB)

 7.12 Rodent Tail Vein Injections (Mice) (PDF 218 KB)

 7.13 Ear Notching for Identification (Mice) (PDF 80 KB)

 7.14 Genotyping (Mice) (PDF 109 KB)

 7.15 Whole Body Perfusion for Small Rodents (Mice) (PDF 159 KB)

 7.17 Acute DSS Colitis (Mice) (PDF 109 KB)

 7.18 Chronic DSS Colitis (PDF 111 KB)

 7.19 Clinical Care of Non-Obese Diabetic (NOD) (Mice) (PDF 123 KB)

 7.20 Manual Restraint of Mice (Mice) (PDF 289 KB)

 7.22 The Supportive Care of Rodents During Research Procedures (Mice) (PDF 127 KB)

 7.23 Rodent Ultrasound (Mice) (PDF 242 KB)

 7.24 Breeding and Weaning (Mice) (PDF 217 KB)

 7.25 Estrus Cycle Monitoring (PDF 903 KB)

 7.26 Intrafemoral Injections (Mice) (PDF 101 KB)

 7.27 Hypoxic Study in Mice (PDF 85 KB)

 14.1 Management of Controlled Substances (PDF 138 KB)

 14.2 Transport of Research Animals on Campus (PDF 119 KB)

 14.3 Clinical Health Reports – Rodent (PDF 116 KB)

 14.4 Rodent Environmental Health Monitoring (PDF 430 KB)

 10.1 Pain Management (Rats) (PDF 157 KB)

 10.2 Humane Interventions (Rats) (PDF 203 KB)

 10.3 Aseptic Surgical Techniques (Rats) (PDF 84 KB)

 10.4 Rodent Post-Operative Care (Rats) (PDF 224 KB)

 10.5.1 Euthanasia of Adult Rodents via Barbiturate or Injectable Anesthetic Overdose (PDF 134 KB)

 10.5.2 Euthanasia of Adult Rodents via CO2 Asphyxiation (PDF 175 KB)

 10.5.3 Euthanasia of Adult Rodents via Overdose of Inhalant Anesthesia (PDF 149 KB)

 10.5.4 Euthanasia of Rodents via Physical Methods (Rats) (PDF 213 KB)

 10.6 Anesthesia (Rats) (PDF 110 KB)

 10.8 Gavage Techniques in Small Animals (Rats) (PDF 184 KB)

 10.9 Injectable Techniques (Rats) (PDF 183 KB)

 10.10 Blood Collection (Rats) (PDF 302 KB)

 10.11 Stereotaxic Surgery (Rats) (PDF 106 KB)

 10.12 Rodent Tail Vein Injections (Rats) (PDF 218 KB)

 10.13 Ear Notching for Identification (Rats) (PDF 90 KB)

 10.14 Genotyping (Rats) (PDF 90 KB)

 10.15 Whole Body Perfusion for Small Rodents (Rats) (PDF 158 KB)

 10.17 Acute DSS Colitis (Rats) (PDF 74 KB)

 10.18 Chronic DSS Colitis (Rats) (PDF 75 KB)

 10.19 Morris Water Maze (Rats) (PDF 205 KB)

 10.20 Manual Restraint (Rats) (PDF 490 KB)

 10.22 The Supportive Care of Rodents During Research Procedures (Rats) (PDF 127 KB)

 10.23 Rodent Ultrasound (Rats) (PDF 242 KB)

 10.24 Breeding and Weaning (Rats) (PDF 207 KB)

 10.25 Estrus Cycle Monitoring (PDF 760 KB)

 14.1 Management of Controlled Substances (PDF 138 KB)

 14.2 Transport of Research Animals on Campus (PDF 119 KB)

 14.3 Clinical Health Reports – Rodent (PDF 116 KB)

 14.4 Rodent Environmental Health Monitoring (PDF 430 KB)

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