Standard Operating Procedures

Standard Operating Procedures (SOPs) provide a detailed description of commonly used techniques. SOPs offer investigators an alternative to writing detailed steps in their Animal Use Protocol (AUP). If referenced within an AUP, any deviation from an SOP must be clearly described and justified in the AUP. Approval of the AUP indicates approval of the deviation for that protocol only.

SOPs are approved by the University Animal Care Committee (UACC).  Refer to the appropriate SOP number and title in the procedural description field within the AUP and include necessary details such as compounds, volumes, frequencies, durations etc. (NOTE: SOPs do not need to be attached to your protocol).

SOPs for commonly used species are listed below. Please follow the link to the Queen's UACC approved SOPs for other species. Access requires registration through the Training Coordinator.

SOP development and revision is an ongoing process. Read all SOPs prior to referencing them in your protocols, as they go through periodic revision. If you require an SOP not currently in the database, contact the University Veterinarian.

For technical assistance, contact the Training Coordinator.

 7.1 Pain Management (Mice) (PDF 82 KB)

 7.2 Humane Interventions (Mice) (PDF 303 KB)

 7.3 Aseptic Surgical Techniques (Mice) (PDF 84 KB)

 7.4 Rodent Post-Operative Care (Mice) (PDF 144 KB)

 7.5.1 Euthanasia of Adult Rodents via Barbiturate or Injectable Anesthetic Overdose (PDF 82 KB)

 7.5.2 Euthanasia of Adult Rodents via CO2 Asphyxiation (PDF 175 KB)

 7.5.3 Euthanasia of Adult Rodents via Overdose of Inhalant Anesthesia (PDF 101 KB)

 7.5.4 Euthanasia of Rodents via Physical Methods (Mice) (PDF 94 KB)

 7.6 Anesthesia (Mice) (PDF 90 KB)

 7.8 Gavage Techniques in Small Animals (Mice) (PDF 148 KB)

 7.9 Injection Techniques (Mice) (PDF 103 KB)

 7.10 Blood Collection (Mice) (PDF 305 KB)

 7.11 Retro-Orbital Injections (Mice) (PDF 156 KB)

 7.12 Rodent Tail Vein Injections (Mice) (PDF 189 KB)

 7.13 Ear Notching for Identification (Mice) (PDF 80 KB)

 7.14 Genotyping (Mice) (PDF 109 KB)

 7.15 Whole Body Perfusion for Small Rodents (Mice) (PDF 154 KB)

 7.17 Acute DSS Colitis (Mice) (PDF 109 KB)

 7.18 Chronic DSS Colitis (PDF 111 KB)

 7.19 Clinical Care of Non-Obese Diabetic (NOD) (Mice) (PDF 72 KB)

 7.20 Manual Restraint of Mice (Mice) (PDF 248 KB)

 7.21 Rodent Weaning (Mice) (PDF 86 KB)

 7.22 The Supportive Care of Rodents During Research Procedures (Mice) (PDF 76 KB)

 7.23 Rodent Ultrasound (Mice) (PDF 138 KB)

 7.25 Estrus Cycle Monitoring (PDF 903 KB)

 7.26 Intrafemoral Injections (Mice) (PDF 101 KB)

 14.1 Management of Controlled Substances (PDF 138 KB)

 14.2 Transport of Research Animals on Campus (PDF 75 KB)

 14.3 Clinical Health Reports – Rodent (PDF 72 KB)

 14.4 Rodent Health Monitoring (PDF 125 KB)

 10.1 Pain Management (Rats) (PDF 82 KB)

 10.2 Humane Interventions (Rats) (PDF 136 KB)

 10.3 Aseptic Surgical Techniques (Rats) (PDF 84 KB)

 10.4 Rodent Post-Operative Care (Rats) (PDF 144 KB)

 10.5.1 Euthanasia of Adult Rodents via Barbiturate or Injectable Anesthetic Overdose (PDF 81 KB)

 10.5.2 Euthanasia of Adult Rodents via CO2 Asphyxiation (PDF 175 KB)

 10.5.3 Euthanasia of Adult Rodents via Overdone of Inhalant Anesthesia (PDF 100 KB)

 10.5.4 Euthanasia of Rodents via Physical Methods (Rats) (PDF 190 KB)

 10.6 Anesthesia (Rats) (PDF 110 KB)

 10.8 Gavage Techniques in Small Animals (Rats) (PDF 102 KB)

 10.9 Injectable Techniques (Rats) (PDF 131 KB)

 10.10 Blood Collection (Rats) (PDF 186 KB)

 10.11 Stereotaxic Surgery (Rats) (PDF 106 KB)

 10.12 Rodent Tail Vein Injections (Rats) (PDF 180 KB)

 10.13 Ear Notching for Identification (Rats) (PDF 90 KB)

 10.14 Genotyping (Rats) (PDF 90 KB)

 10.15 Whole Body Perfusion for Small Rodents (Rats) (PDF 154 KB)

 10.17 Acute DSS Colitis (Rats) (PDF 74 KB)

 10.18 Chronic DSS Colitis (Rats) (PDF 75 KB)

 10.19 Morris Water Maze (Rats) (PDF 133 KB)

 10.20 Manual Restraint (Rats) (PDF 472 KB)

 10.22 The Supportive Care of Rodents During Research Procedures (Rats) (PDF 76 KB)

 10.23 Rodent Ultrasound (Rats) (PDF 138 KB)

 10.24 Breeding and Weaning (Rats) (PDF 116 KB)

 10.25 Estrus Cycle Monitoring (PDF 760 KB)

 14.1 Management of Controlled Substances (PDF 138 KB)

 14.2 Transport of Research Animals on Campus (PDF 75 KB)

 14.3 Clinical Health Reports – Rodent (PDF 72 KB)

 14.4 Rodent Health Monitoring (PDF 125 KB)

animal facility

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